Skip to main content
. 2002 Apr;22(8):2586–2597. doi: 10.1128/MCB.22.8.2586-2597.2002

FIG. 8.

FIG. 8.

Smad-2, MAPK, and AKT phosphorylation by CR-1ΔC in EpH-4 WT and EpH-4 N cells transiently transfected with a dominant-negative ALK4-2-Flag receptor. EpH-4 WT and EpH-4 N cells transiently transfected with ALK4-2-Flag or pCI-neo plasmid were stimulated with CR-1ΔC, EGF, or Activin A recombinant protein and cell lysates were assessed by Western blot (WB) analysis. (A) EpH-4 N cells transfected with pCI-neo (4 μg) (lanes 1, 2, and 4) and ALK4-2 (4 μg) were stimulated with Activin A (50 ng/ml) and CR-1ΔC (400 ng/ml) and analyzed by Western blot analysis. (B and C) EpH-4 WT or EpH-4 N cells transfected with pCI-neo (6 μg) (lanes 1, 2, and 4) and ALK4-2 (6, 4, 2, and 1 μg) were stimulated with EGF (50 ng/ml) and CR-1ΔC (200 ng/ml) and analyzed by Western blot analysis. MW, molecular weight in thousands; C, control.