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MAPK and AKT phosphorylation by CR-1ΔC in MC3T3-E1 cells. (A) RT-PCR using specific primers for ALK4 (435 bp) in EpH-4 WT and MC3T3-E1 cells. A 100-bp DNA marker is shown in the first lane. (B and C) Serum-starved MC3T3-E1 cells were stimulated with CR-1ΔC (200 and 400 ng/ml) or EGF (50 ng/ml) recombinant protein and analyzed by Western blot (WB) analysis. MW, molecular weight in thousands; C, control.
