Fig. 6.

JAK2/STAT3 pathway acts as IL7R downstream involved in miR-3614-5p mediated CRC suppression. (A) Western blot was used to assess the expression levels of p-JAK2 and p-STAT3 in CRC cells with or without miR-3614-5p mimics or corresponding control. (B) Western blot was used to assess the expression levels of p-JAK2 and p-STAT3 in CRC cells with IL7Rα plasmid or corresponding control. (C-E) CCK8, wound healing, and transwell assays were performed to evaluate the effect of AG490 on the proliferation, migration, and invasion of CRC cells mediated by IL7Rα and miR-3614-5p. Scale bars = 100 μm (D) and 100 μm (E). *p < 0.05