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. 2002 Apr;22(8):2505–2514. doi: 10.1128/MCB.22.8.2505-2514.2002

FIG. 1.

FIG. 1.

Verification of the cloning of BP1. (A) Binding characteristics of the BP1 protein. The probe was incubated with fusion protein, as described in Materials and Methods, in the absence or presence of cold competitor oligonucleotide. The band at the top marks the position of the wells, and the band at the bottom contains the free probe. The arrow indicates the position of the shifted band. Per reaction, 10,000 cpm of probe was used, and the amount of cold competitor added was based on the specific activity of the probe. Lanes 2, 4, 6, 8, and 10 contain a 100× excess of the cold competitor indicated, and lanes 3, 5, 7, 9, and 11 contain a 200× excess of cold competitor. (B) Antibody against the cloned BP1 protein (BP1-Ab) recognizes the BP1 protein in a nuclear extract. Bands c and d represent specific shifted bands, and bands a and b are supershift bands. The amount of competitors were 500× self DNA (lane 2) and 500× nonspecific (N.S.) DNA (lane 3). +, present; −, absent.