FIG. 4.

Proteins from K562 cells and BP1 in vitro protein give a common shifted band with the EMSA. Proteins from K562 cells or BP1 in vitro protein translated in a wheat germ system were incubated with a probe containing the BP1 binding site in silencer I. The free probe is at the bottom. Cold DNA competitors, added at 1,000× excess, were the Indian haplotype sequence (lanes 2 and 6) or nonspecific (N.S.) DNA (lanes 3 and 7). Lanes 1 and 5 are controls lacking competitors, and lane 4 contains only wheat germ extract (W.G.) as a control.