TABLE 1.

Quantitative analysis of the ratio between the long and short axes of HeLa cells expressing Nir2 and its truncated mutants^a

Cell type	Long axis (μm)	Short axis (μm)	Ratio (L/S)
Control	46.75 ± 0.40	29.33 ± 0.31	1.61 ± 0.02
ΔPI	82.81 ± 0.49	30.30 ± 0.14	2.75 ± 0.02
ΔPB	83.49 ± 0.51	30.39 ± 0.14	2.88 ± 0.02
ΔN1	61.43 ± 0.27	30.22 ± 0.07	2.02 ± 0.01
ΔN2	48.35 ± 0.16	29.49 ± 0.14	1.70 ± 0.01
Nir2	84.96 ± 0.51	29.07 ± 0.16	2.96 ± 0.02

^aQuantitative analysis of the ratio between the long and short axes of HeLa cells expressing Nir2 and its truncated mutants. HeLa cells were either mock transfected (control) or transfected with expression vectors encoding the different Nir2 truncated mutants as indicated. After 24 h, the cells were immunostained with anti-HA antibodies and analyzed by fluorescence microscopy. The lengths of the long and short axes of 30 to 50 cells expressing the indicated protein were measured by Zeiss 510 confocal microscope operating software (version 2.01). Shown are the means ± standard errors of the means of the long and short axes along with the calculated ratio between them (L/S).