TABLE 1.
Primers used for genotyping, bisulfite analysis, and reverse transcription-PCR
| Primer no. (name)a | H19 position no.b | Sequence (5′-3′) |
|---|---|---|
| G1 (H19-3.9F) | −3953 (1303) | TGCAAGGAGACCATGCCTATTCTTG |
| G2 (H19-2.3F) | −2286 (2971) | CAATGTTCATAAGGGTCATGGGGTG |
| G3 (PGK-neoF) | NAc | CCACTTGTGTAGCGCCAAGTGCC |
| G4 (H19-4.8F) | −4811 (445) | CCAACTGAGAGGGCCATAGTGTGAG |
| G5 (H19-2.0R) | −2031 (3227) | CGTAAAGTGTCACAAATGCCTGATCCC |
| G6 (HSEQ1R) | −750 (4551) | CCACAGAGTCAGCATCCAC |
| B1 (BMsp2t1) | −3978 (1278) | GAGTATTTAGGAGGTATAAGAATT |
| B2 (BMsp2t2) | −3952 (1304) | GTAAGGAGATTATGTTTATTTTTGG |
| B3 (BHhalt4) | −3532 (1726) | CCTCATTAATCCCATAACTAT |
| B4 (BHhalt3) | −3507 (1751) | ATCAAAAACTAACATAAACCCCT |
| B5 (BHha7t) | −2031 (3225) | TAAAATATCACAAATACCTAATCCCT |
| B6 (BHSEQm1) | −709 (4551) | CCATTACTCTTAACTTCTATTAAA |
| B7 (BHSEQm2) | −733 (4527) | AATTATACAACTAAAATCCACAAAATCAAC |
| B8 (BTV3-1) | −2310 (2938) | GGTAAATTTATGGGTTATTTAAGG |
| B9 (BTV3-2) | −2286 (2972) | AATGTTTATAAGGGTTATGGGGTGG |
| B10 (BTV3-3) | −1861 (3397) | CCTAAATTCAATAAAACATTACAA |
| B11 (BTV3-4) | −1884 (3374) | CCCAACCTCTACTTTTATAAC |
| RT1 (HI3) | +1722 (7242)d | CCTCAAGATGAAAGAAATGGT |
| RT2 (HE5) | +2528 (8048)d | AACACTTTATGATGGAACTGC |
a
G, genotyping; B, bisulfite analysis; RT, reverse transcription-PCR.
b
Specifies the position of the first nucleotide of the primer relative to the H19 transcription start site (position in parentheses according to GenBank accession no. U19619, unless otherwise noted).
c
NA, not applicable, primer for neoR sequence.
d
Position in parentheses according to GenBank accession no. AF049091.