TABLE 2.
Activation of paternal H19 and maternal Igf2 expression from mutant alleles
| Tissue type | Deletion | Mutant paternal/wild-type maternal H19 ratioa | Mutant maternal/wild-type paternal Igf2 ratioa |
|---|---|---|---|
| Liver | ΔDMD | 0.51 ± 0.03 (6)b | 0.29 ± 0.06 (27) |
| Δ3.8kb-5′H19 | 0.53 ± 0.02 (6)b | 0.30 ± 0.05 (6) | |
| Tongue | ΔDMD | 0.37 ± 0.02 (6)b | 0.52 ± 0.07 (8) |
| Δ3.8kb-5′H19 | 0.32 ± 0.04 (6)b | 0.56 ± 0.09 (6) | |
| Kidney | ΔDMD | <0.06 ± NA (4)b | 0.51 ± 0.06 (6) |
| Δ3.8kb-5′H19 | <0.06 ± NA (6)b | 0.68 ± 0.09 (6) | |
| Heart | ΔDMD | 0.10 ± 0.022 (3)b | 0.62 ± 0.12 (6) |
| Δ3.8kb-5′H19 | 0.14 ± 0.014 (3)b | 0.77 ± 0.22 (5) | |
| Lung | ΔDMD | 0.14 ± 0.07 (3)b | 0.54 ± 0.07 (6) |
| Δ3.8kb-5′H19 | 0.23 ± 0.16 (3) | 0.96 ± 0.06 (3) | |
| Brain | ΔDMD | 0.38 ± 0.05 (3)b | 0.94 ± 0.28 (3)c |
| Δ3.8kb-5′H19 | 0.48 ± 0.05 (3) | NDd | |
| Gut | ΔDMD | 0.19 ± 0.09 (3)b | 0.35 ± 0.04 (9) |
| Δ3.8kb-5′H19 | 0.19 ± 0.06 (3) | NDd |
a
Numbers are ratios of expression from the mutant to wild-type allele averaged for number of animals assayed ± standard deviation. Values in parentheses represent the number of animals assayed. H19 expression levels were measured by LightCycler Real-Time PCR, and Igf2 expression levels were measured by RPA. In wild-type control samples, there was no observed activation of paternal H19 and no or very low (<0.04 maternal/paternal ratio) levels of activation from the maternal Igf2.
b
A similar level of expression was also measured by RPA (Fig. 3A).
c
Biallelic expression was detected in both wild-type and mutant littermates.
d
ND, not determined.