TABLE 3.

Reduction of maternal H19 and paternal Igf2 expression from mutant alleles

Tissue type	Deletion	Mutant maternal/wild-type maternal H19 ratioa	Mutant paternal/wild-type paternal Igf2 ratioa
Liver	ΔDMD	0.44 ± 0.05, 0.41 ± 0.11	0.28 ± 0.04, 0.35 ± 0.03
	Δ3.8kb-5′H19	0.26 ± 0.05, 0.37 ± 0.03	0.26 ± 0.05, 0.38 ± 0.10
Tongue	ΔDMD	0.59 ± 0.12	0.75 ± 0.12
	Δ3.8kb-5′H19	0.53 ± 0.06	0.71 ± 0.06
Kidney	ΔDMD	≤0.10	∼1.0b
	Δ3.8kb-5′H19	≤0.10	∼1.0b
Heart	ΔDMD	0.26 ± 0.04	∼1.0b
	Δ3.8kb-5′H19	0.15 ± 0.05	∼1.0b
Lung	ΔDMD	0.11 ± 0.03	∼1.0b
	Δ3.8kb-5′H19	≤0.10	∼1.0b
Brain	ΔDMD	0.56 ± 0.13	∼1.0b
	Δ3.8kb-5′H19	0.53 ± 0.11	NDc

^aExpression was standardized to rpL32 and averaged in 2 or 3 wild-type and 2 or 3 mutant littermates. Results from one or two litters are presented. Numbers are the ratios of the averaged mutant to averaged wild-type level of expression as detected by RPA and/or Northern analysis ± deviation. The noted deviations reflect the variation between the independent mutant and the independent wild-type samples that were assayed. There is no paternal H19 and maternal Igf2 expression from the wild-type (CAST) alleles.

^bNo effect was detected; wild-type and mutant paternal Igf2 RNA levels were similar.

^cND, not determined.