FIG. 6.

Polyribosome profiles from a dom34Δ rps30AΔ strain show similar 80S monoribosome increases to those for a hbs1Δ rps30AΔ strain. Wild-type yeast (CP1) or cells lacking dom34Δ (TKY525), hbs1Δ dom34Δ (TKY514), dom34Δ rps30AΔ (TKY522), and dom34Δ hbs1Δ rps30AΔ (TKY535) were grown at 30°C to mid-log phase. Cells were extracted or shifted to 13°C for 2 h prior to extraction. Polyribosome content was analyzed on a 7 to 47% sucrose gradient, and subunits and ribosomes are as indicated in the first panel.