Figure 2.

Performance metrics of modalities. (a) Sensitivity, as illustrated for each donor using the relationship between median genes per cell and median reads per cell for the five modalities (sequencing depth shown for each modality is to the manufacturers minimum recommendation +5%, Supplementary Fig. S1 presents the two donors together for each modality seperately). (b) Sensitivity for STBs, as illustrated for each donor using the relationship between average transcripts per cell and reads per cell for the five modalities. (c) Sensitivity for Natural Killer/T cells (NK/T), as illustrated for each donor using the relationship between average transcripts per cell and reads per cell for the five modalities. (d) Capture efficiency, showing the percentage of cells used for analysis post Quality Control filtering in relation to cells inputted for each modality. (e) Background, illustrating per modality the fraction of reads removed by the computational tool used for removing reads assigned to ambient RNA.