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ATP regulates interaction between SODD and TNFR1. GST-SODD1-457 was immobilized on glutathione-Sepharose and incubated with lysates of COS-7 cells expressing endogenous HSC70 and transfected FLAG-TNFR1211-425 in the presence of ATP at the indicated concentrations (A) or of no nucleotide or 1 mM concentrations of ATP or other nucleotides (B). Binding proteins were analyzed as described in the legend to Fig. 1. precipitate.
