TABLE 2.
Regulated genes identified in microarray experimentsa
| ORF | Gene | Gof | Yrr1S*GAD | Function | Promoter region analyzed | Group | EMSA | PDRE | YRRE | Drug resistance |
|---|---|---|---|---|---|---|---|---|---|---|
| YGR224W | AZR1 | 29 | 16.2 | Protein involved in resistance to azoles and acetic acid; member of the MFS MDR protein family | −271/−93 | 1 | 1 | 1 | Acetic acid, ketoconazole, and fluconazole | |
| YLR046C | YLR046C | 12.6 | 8.8 | Protein with similarity to Rtm1p | −442/−267 | 1 | NS | 0 | ||
| YLL056C | YLL056C | 11.2 | 10.5 | Protein with weak similarity to Yersinia pseudotuberculosis CDP-3, 6-dideoxy-d-glycero-l-glycero-4-hexulose-5-epimerase | −348/−182 | 1 | 1 | 1 | 1 | |
| YBR008C | FLR1 | 3.7 | 2 | Member of the MDR 12-span (DHA12) family of MFS-MDR | −471/−299 | 1 | 1 | 1 | 2 | Fluconazole, 4-NQO, benomyl, methotrexate, fluconazole, and cycloheximide |
| YPL088W | YPL088W | 2.5 | 5.2 | Putative aryl alcohol dehydrogenase, may participate in late steps of degradation of aromatic compounds that arise from the degradation of lignocellulose | −357/−212 | 1 | 1 | 1 | 3 | |
| YLR179C | YLR179C | 2 | 1.7 | Protein with similarity to Tfs1p Cdc25p-dependent nutrient- and ammonia-response protein | −397/−200 | 1 | NS | 0 | ||
| YGR197C | SNG1 | 5 | 3.1 | Probable transport protein, confers resistance to MNNG and nitrosoguanidine | −434/−248 | 1 | 1 | 1 | MNNG | |
| YDR011W | SNQ2 | 3.2 | 1.5 | Drug-efflux pump involved in resistance to multiple drugs, member of the ABC-transporter superfamily | −693/−503 | 2 | 1 | 4 | 4 | 4-NQO, fluphenazine, azole antifungal agents, staurosporin, cercosporin, and clotimazol Copper |
| YBR151W | APD1 | 2.6 | 2.1 | Protein required for normal cellular structure, localization of actin patches, and resistance to copper | −344/−174 | 2 | 1 | 2 | ||
| YMR008C | PLB1 | 1.8 | 1.7 | Phospholipase B, preferentially deacylates phosphatidylcholine and phosphatidylethanolamine | ? | 2 | ? | 2 | ||
| YGR035C | YGR035C | 1.9 | 7.1 | Protein of unknown function | −430/−242 | 3 | NS | 2 | 3 | |
| YMR102C | YMR102C | 1.4 | 2.9 | Protein of unknown function, contains WD (WD-40) repeats | −770/−577 | 3 | NF | 2 | 2 | |
| YKL051W | YKL051W | 1.9 | 2.9 | Protein of unknown function | −639/−469 | 3 | NF | 2 | ||
| YGR281W | YOR1 | 2.3 | 5.7 | Oligomycin resistance factor; member of the ABC superfamily | −500/−318 | ? | 1 | 1 | 2 | Oligomycin, staurosporin, azole antifungal agents, tetracycline, and erythromycin |
| YLR346C | YLR346C | 3.7 | 2.8 | Protein of unknown function | −388/−221 | ? | 2 | 1 | 2 |
This table provides information concerning the 15 regulated genes identified in experiments with the gain-of-function mutant and the Yrr1S∗GAD chimera. The mean expression ratio was determined by microarray analysis for the gain-of-function (Gof) mutant and the short chimera (Yrr1S∗GAD). Each value is the mean for six independent arrays. Gene function is given according to YPD definition (3). The results of promoter analysis are also given. We also provide details of the location of the promoter region used for EMSA analysis. The group number according to Northern blot kinetic analysis is indicated. We also indicate the number of significant shifts seen in Fig. 3, nonspecific mobility shifts (NS), and the region for which no shift was seen (NF). We also give the number of PDR elements (PDRE) and Yrr1p response elements (YRRE) present in the promoter region of each target. Drug resistance is included for each gene for which it has been characterized. MNNG, N-methyl-N′-nitro-N-nitrosoguanidine ORF, open reading frame.