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JBD does not inhibit MEKK1-mediated activation of PSA. LNCaP cells were transfected with empty vector pcDNA3, JBD (500 ng), MEKK1-DA (400 ng), or JBD with MEKK1-DA, together with the reporter construct driven either by a Jun-responsive element (Jun-Luc; 200 ng) (A) or by PSA-P/E-Luc (200 ng) (B). Luciferase activity was measured 2 days after transfection and normalized by transfection efficiency, which was determined by GFP cotransfection. The data shown represent three experiments.
