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. 2002 Apr;22(8):2862–2870. doi: 10.1128/MCB.22.8.2862-2870.2002

FIG. 2.

FIG. 2.

Dominant-active IκBα mutant inhibits MEKK1-mediated activation of PSA. LNCaP cells were transfected with empty vector pcDNA3, the mutant form IκBα(32/36AA) (800 ng), MEKK1-DA (300 ng), or MEKK1-DA with increasing amounts (100, 200, 400, and 800 ng) of the mutant form IκBα(32/36AA), together with the reporter construct driven either by an NF-κB-responsive element (NF-κB-Luc; 200 ng) (A) or by PSA-P/E-Luc (200 ng) (B and D). (C) Lysates from the transfected cells were examined for MEKK1-DA expression. Bicalutamide was used as indicated in the experiment whose results are shown in panel D. Luciferase activity was measured 2 days after transfection and normalized by transfection efficiency, which was determined by GFP cotransfection. The data shown represent three experiments.