FIG. 3.
TNF-α activates PSA expression through the NF-κB pathway. LNCaP cells were transfected with the reporter construct PSA-P/E-Luc (200 ng) with or without the mutant form IκBα(32/36AA) (800 ng) as indicated. After transfection, cells were treated with or without TNF-α (10 or 50 ng/ml) as indicated. Luciferase activity was measured 2 days after transfection and normalized to Renilla luciferase. The data shown represent two experiments.