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TNF-α activates PSA expression through the NF-κB pathway. LNCaP cells were transfected with the reporter construct PSA-P/E-Luc (200 ng) with or without the mutant form IκBα(32/36AA) (800 ng) as indicated. After transfection, cells were treated with or without TNF-α (10 or 50 ng/ml) as indicated. Luciferase activity was measured 2 days after transfection and normalized to Renilla luciferase. The data shown represent two experiments.
