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Setting up enrichment cultures with environmental microbial communities growing on plastic additives. (a–d) Environmental sampling locations. (Chessel Bay, Swansea Bay, Wastewater Plant, and Recycling Plant from left to right). (e–h) Sampling, sorting, collecting, and culturing microbial inocula in deep-well microplate enrichment cultures. (g) Inocula in same order (left to right) as (a)–(d), after addition of buffer to sorted plastic particles.
