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. 2026 Jan 28;3(1):qvag003. doi: 10.1093/sumbio/qvag003

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© The Author(s) 2026. Published by Oxford University Press on behalf of Applied Microbiology International.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time-course profiles of four biochemical assays measured across substrate treatments and inoculum sources. Lines represent mean values (N = 4) for each inoculum (see legend), for each substrate (columns: NC, DEHT, tetradecane) at day 2, 7, and 22, shaded areas indicate ± standard deviation (SD). Each row shows a different assay: total biomass (SYBR Green I, SGI assay in AU), dead cells (PI assay in AU), redox activity (2,6-dichlorophenolindophenol, DCPIP assay in %), and esterase activity (Fluoroscein dilaurate, FDL assay with PLA in AU). Temporal trends for each substrate in each assay between sequential sampling points (days 2–7 and 7–22) are indicated by arrows (↑ increase, ↓ decrease) and significance markers (* for P < .05, ** for P < .01, and *** for P < .001), based on pairwise comparisons across all inocula, adjusted for multiple testing.