Figure 3.

Diversity of enriched microbial communities is shaped by environmental inocula and substrate. (a) Alpha diversity (Chao1) of microbial communities across inocula and substrates. Boxplots show the estimated number of ASVs for each inoculum and substrate combination; there are three biological replicates for each combination, each of which was prepared and sequenced four times (four technical replicates). Facets correspond to different inoculum sources. Asterisks indicate pairwise Wilcoxon test significance levels between substrates within each inoculum (*P < .05, **P < .01, ***P < .001; BH-adjusted*). Error bars show interquartile ranges, with the midline representing the median. (b) NMDS using Bray–Curtis distance on Hellinger transformed bacterial community structure at ASV level (957 ASVs in 142 samples). Points represent individual technical replicates (N = 4) for each biological replicate (N = 3). Data for each inocula are shown separately, on the same axes, colored by substrate. Ellipses indicate 95% confidence intervals for each substrate group within an inoculum.