FIG. 6.

Analysis of HeT-A transcripts in the Su(var)2-5 mutant strains. (A) Northern blot hybridization with total RNA isolated from progeny from the In(I)w^m4h; Su(var)2-5⁰⁴/In(2L)^tIn(2R)Cy[Su(var)04/Cy], Df(1)w, and y¹w^67c23; Su(var)2-5⁰²/CyO y⁺[Su(var)02/Cy]; Oregon R lines and the In(I)w^m4h; Su(var)2-5⁰⁵/In(2L)Cy¹ In(2R)Cy[Su(var)05/Cy]; Gaiano and yac lines. The double-strand probe from the open reading frame of HeT-A detects a prominent RNA of ∼6 kb. The probe from the ras2 gene gives rise to a 1.6-kb transcript that is used as a marker for the amount of RNA. (B) Northern blot hybridization with total RNA isolated from flies obtained in the crosses is depicted in the lower part of the figure. Gaiano and If/CyO lines were used as controls with high and low HeT-A transcription, respectively. The In(I)w^m4h; Su(var)2-5⁰¹/SM1 [Su(var)01/Cy] and In(I)w^m4h; Su(var)2-5⁰⁴/In(2L)^tIn(2R)Cy Cy¹Roi¹pR¹cn¹[Su(var)04/Cy] lines were used in the crosses.