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Effect of overexpression of NRR on CAD activity. In Hep3B cells, pFR-Luc (1 μg) and pRL-CMV (0.1 μg) were cotransfected with either GH1α740-826 (1 μg) (A) or GH1α786-826 (B). In addition, the indicated amount (in micrograms) of empty vector, plasmids expressing HIF-1α740-813, or VP16 was cotransfected. Luciferase assays were performed 48 h after transfection, and the luciferase activity (RLU) was corrected by cotransfected pRL-CMV.
