Computer-assisted analysis of sperm motility. The results of analyses of wild-type (▪) and Smcp−/− (□) spermatozoa are shown. Sperm velocities (micrometers/second), forward movement (percent), lateral amplitude of the sperm head (micrometers), and beat frequency (hertz) were measured after 1.5, 3.5, and 5.5 h. For velocities (VAP, VSL, and VCL) and the beat frequency (BCF), the means and appropriate standard deviations are illustrated. For amplitude (ALH) and straightness (STR), the medians and quartiles are shown. The Smcp-deficient spermatozoa exhibit highly significantly reduced velocities, beat frequency, amplitude and forward movement in comparison to wild-type sperm (P < 0.001). (A) VAP; (B) VSL; (C) VCL; (D) BCF; (E) ALH; (F) STR.