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. 2002 May;22(9):3157–3173. doi: 10.1128/MCB.22.9.3157-3173.2002

FIG. 7.

FIG. 7.

Diffusion of methylation-dependent silencing is blocked by exogenous GAL4VP16 but not TBP. GAL4 (lane 3), GAL4VP16 (lane 4), or TBP (lane 5) mRNA was injected into stage VI oocytes. After 16 h, a mixture of templates containing the regionally methylated 11-aprtGal4×5-tkCAT and CMVCAT DNAs was injected (lanes 2 to 5). In lane 1, the injected DNA mixture contained the mock-methylated vector together with CMVCAT. Oocytes were incubated for 16 h, and RNA was extracted and analyzed by primer extension. The upper part of the figure shows a scheme of the regionally methylated 11-aprtGal4×5-tkCAT template.