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. 2002 May;22(10):3281–3291. doi: 10.1128/MCB.22.10.3281-3291.2002

FIG. 3.

FIG. 3.

DNA binding and ATPase activities of the Mer3 protein. Binding of Mer3 protein to oligonucleotide substrates was detected by gel mobility shift assays as described in Materials and Methods. Amounts of the Mer3 or Mer3GD protein are indicated. (A) Binding of the Mer3 protein to 50-nt ssDNA and 50-bp dsDNA. (C) Binding of the Mer3GD protein to ssDNA and dsDNA. (E) Binding of the Mer3 protein to dsDNA, with or without boiling of the DNA for 3 min prior to addition of the protein. The percentage of labeled DNA present in protein-DNA complexes in reactions is plotted in panels B (dsDNA or ssDNA with Mer3), D (dsDNA or ssDNA with Mer3GD), and F (dsDNA or boiled dsDNA with Mer3). The value plotted is the mean of the values obtained in at least two experiments, and the bar shows the standard deviation. (G, H, and I) ATPase activity of Mer3, Mer3GD, and Mer3KA proteins in the absence of additional DNA and in the presence of poly(dA) or M13mp18 replication form I (M13 RF) was examined by thin-layer chromatography as described in Materials and Methods.