TABLE 3.
Frequency of crossing over in mer3 mutant strains
Relevant genotype | Interval | Result ata:
|
|||||||
---|---|---|---|---|---|---|---|---|---|
30°C
|
23°C
|
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No. of events
|
Map distance (cM) | No. of events
|
Map distance (cM) | ||||||
PD | TT | NPD | PD | TT | NPD | ||||
MER3 | CAN1-URA3 | 206 | 312 | 15 | 38 | 204 | 316 | 17 | 39 |
URA3-HOM3 | 217 | 306 | 11 | 35 | 195 | 325 | 18 | 40 | |
HOM3-TRP2 | 438 | 94 | 2 | 10 | 438 | 95 | 1 | 9.5 | |
mer3GD | CAN1-URA3 | 313 | 214 | 9 | 25 (0.66) | 325 | 192 | 3 | 20 (0.51) |
URA3-HOM3 | 346 | 191 | 5 | 20 (0.57) | 359 | 159 | 3 | 17 (0.43) | |
HOM3-TRP2 | 428 | 102 | 5 | 12 (1.2) | 433 | 83 | 1 | 8.6 (0.91) | |
mer3KA | CAN1-URA3 | 336 | 205 | 5 | 22 (0.58) | 337 | 177 | 10 | 23 (0.59) |
URA3-HOM3 | 352 | 183 | 5 | 20 (0.57) | 377 | 146 | 3 | 16 (0.40) | |
HOM3-TRP2 | 447 | 81 | 3 | 9.3 (0.93) | 436 | 83 | 2 | 9.1 (0.96) |
Tetrads obtained in Table 2 were examined. Only 4-sv tetrads that did not show aberrant segregation of the relevant markers were used to calculate map distances. Numbers in parentheses show relative frequencies of crossing over in the mer3GD and mer3KA mutants compared to those in the corresponding intervals in the wild-type strain. PD, parental ditype; TT, tetratype; NPD, nonparental ditype.