Skip to main content
. 2002 May;22(10):3316–3326. doi: 10.1128/MCB.22.10.3316-3326.2002

FIG. 4.

FIG. 4.

Kinetics of Jak2 ubiquitination and degradation in vivo. Jak2WT-HA (A) and Jak2KN-HA (B) were transiently expressed in Cos-7 cells. After 48 h the cells were starved overnight and stimulated with pervanadate (50 μM) as indicated. Right blots (A and B), lysates from cells pretreated with proteasome inhibitor MG132 (25 μM) for 1 h before pervanadate treatment. Jak2 was assessed by immunoblotting using an antiubiquitin or antiphosphotyrosine (anti-PTyr) antibody followed by stripping and anti-HA blotting. The antiubiquitin and antiphosphotyrosine blots for Jak2WT-HA and Jak2KN-HA were exposed for the same time. IP, immunoprecipitation.