Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 May;22(10):3316–3326. doi: 10.1128/MCB.22.10.3316-3326.2002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, American Society for Microbiology

PMC Copyright notice

FIG. 4. — Kinetics of Jak2 ubiquitination and degradation in vivo. Jak2WT-HA (A) and Jak2KN-HA (B) were transiently expressed in Cos-7 cells. After 48 h the cells were starved overnight and stimulated with pervanadate (50 μM) as indicated. Right blots (A and B), lysates from cells pretreated with proteasome inhibitor MG132 (25 μM) for 1 h before pervanadate treatment. Jak2 was assessed by immunoblotting using an antiubiquitin or antiphosphotyrosine (anti-PTyr) antibody followed by stripping and anti-HA blotting. The antiubiquitin and antiphosphotyrosine blots for Jak2WT-HA and Jak2KN-HA were exposed for the same time. IP, immunoprecipitation.