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. 2002 May;22(10):3316–3326. doi: 10.1128/MCB.22.10.3316-3326.2002

FIG. 6.

FIG. 6.

Proteasome inhibitor MG132 stabilizes the interaction between Jak2WT and SOCS-1. (A) Coimmunoprecipitation of Jak2 and SOCS-1. Cos-7 cells were transfected with Jak2WT-HA and SOCS-1 as indicated and treated with MG132 and pervanadate as described for Fig. 5. Total-cell lysates were immunoprecipitated (IP) with anti-HA or anti-SOCS-1 antibodies and analyzed by immunoblotting using anti-SOCS-1 or anti-HA antibodies. (B) Coimmunoprecipitation of SOCS-1 with different Jak2 mutants. Cos-7 cells were transfected with Jak2WT-HA (lanes 1 and 2), Jak2KN-HA (lanes 3 and 4), and Jak2YF-HA (lanes 5 and 6) together with SOCS-1. Cells were pretreated with MG132 (25 μM) for 1 h before the pervanadate treatment (25 μM). After anti-HA immunoprecipitation, the filter was immunoblotted with an anti-SOCS-1 antibody.