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SOCS-1 enhances the ubiquitination of Jak2WT in vitro. Immunopurified Jak2WT, Jak2YF, and Jak2KN were subjected to an in vitro ubiquitination reaction in the presence SOCS-1, SOCS-1ΔSB, or control lysates. The ubiquitination of Jak2 was analyzed after 1 h of in vitro reaction by antiubiquitin Western blotting. The filter was stripped and reblotted with an anti-Jak2 antibody.
