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Excess Pab1p acts against phenotypic suppression. Strain 33G-D373 was transformed with pYX242 and pYX242/PAB1 plasmids. Drops of yeast suspensions with the same cell concentration were plated on selective medium with paromomycin and incubated at 25°C. The His+ phenotype indicated phenotypic suppression, and the Leu+ phenotype confirmed the plasmid transformation. Ten independent transformants with each plasmid were tested. Representative results are shown.
