TABLE 3.
Cell cycle distribution (%) of A549 transfected with or without various expression constructsa
Treatment | % Found at different phases
|
||
---|---|---|---|
G1 | S | G2/M | |
A549 | 43 | 38 | 19 |
Vector | 47 | 36 | 18 |
Plk3-A | 33 | 36 | 32 |
Plk3K52R | 38 | 35 | 28 |
A549 + taxol | 35 | 39 | 26 |
Vector + taxol | 34 | 35 | 30 |
Plk3-A + taxol | 26 | 40 | 33 |
Plk3K52R + taxol | 29 | 38 | 33 |
Parental A549 cells or A549 cells transfected with Plk3-A, Plk3K52R, or the vector alone for 12 h were treated with or without taxol for an additional 4 h. The cells were then processed and stained with propidium iodide. Cell cycle distributions were analyzed by flow cytometry. Data were summarized from three independent experiments.