TABLE 3.
Functional categorization of recombination-defective mutants
| Group | Mutant | Defect |
|---|---|---|
| 1 | K405/H406/R407 | Impaired DNA binding, nicking, and hairpin formation |
| R748/H750 | ||
| R773/R775 | ||
| H937/K938 | ||
| H942 | ||
| R969/R970 | ||
| K966 | ||
| R977 | ||
| 2 | K596 | Impaired nicking and hairpin formation |
| R621 | ||
| R713a | ||
| R734 | ||
| H795a | ||
| K989/H990/H991 | ||
| 3 | R855/K856b | Impaired hairpin formation |
| K890b | ||
| R894b | ||
| R972/K973 | ||
| 4 | K608 | Conditionally defective in hairpin formation (sensitive to coding flank sequence) |
| 5 | R401/R402c | Impaired joining |
| R440c | ||
| R838/K839/R840d | ||
| K980d |
a
Cannot form hairpins even on a prenicked substrate.
b
Also tends to form aberrant hairpins.
c
Defective for both coding joint and signal joint formation.
d
More severely defective for coding joint than for signal joint formation.