Skip to main content
. 2002 May;22(10):3389–3403. doi: 10.1128/MCB.22.10.3389-3403.2002

FIG. 2.

FIG. 2.

FIG. 2.

FIG. 2.

Activation of p38 by active MKK3 or MKK6 induces premature senescence. (A) Western blot analysis of BJ cells transduced at PD 18 with a vector control (BP) or constitutively active MKK3 (MKK3E) or MKK6 (MKK6E). Levels of MKK3, MKK6, phospho-ERK (P-ERK1/2), ERK2, phospho-p38 (P-p38), p38, p16INK4A, and p53 were determined 10 days postinfection. (B) PD of BJ cells between day 7 and day 11 after infection at PD 18 with a vector control (BP), Ha-RasV12 (HaRasV12), a constitutively active MKK3 (MKK3E) or MKK6 (MKK6E), or wild-type p38α (p38αWT). Values are means ± standard deviations (SD) for triplicates. (C) Morphology of BJ cells (BJ) or BJ cells immortalized with hTERT (BJ-hTERT) that had been transduced with a vector control (BP) or constitutively active MKK3 (MKK3E) or MKK6 (MKK6E), after staining for SA-β-gal (pH 6.0) at day 12 postinfection. (D) Quantification of percentages of SA-β-gal-positive cells within cell populations shown in panel C. Values are means ± SD for two separate wells. At least 200 cells were counted for each sample.