FIG. 5.
Activation of JNK by active MKK7 does not induce senescence. (A) Levels of phospho-JNK (P-JNK), JNK1, phospho-p38 (P-p38), p16INK4A, and actin were determined by Western blot analysis 10 days after transduction of BJ cells at PD 24 with Ha-RasV12 (HaRasV12), constitutively active MKK7 (MKK7D), or a vector control (BP). (B) PD of BJ cells transduced at PD 24 with Ha-RasV12 (Ras), constitutively active MKK7 (MKK7D), or a vector control (BP). Day 0 represents the fifth day postinfection. Values are means ± standard deviations for triplicates.