FIG. 3.

Synergistic activation of an NF-κB-responsive promoter by SRC-3 and IKK. HeLa cells were transfected with either SRC-3 or IKK expression vector alone or with the two combined. Where indicated, TNF-α (+) was added 24 h after transfection for 4 h. Thereafter, the luciferase activity derived from the transfected κB-responsive promoter was measured and normalized by total input proteins. The y axis represents relative activities by comparison to those of samples transfected with the NF-κB-responsive promoter alone, whose activity was assigned a value of 1. The bars represent the means and standard deviations of the results from three independent experiments with triplicate samples.