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. 2002 May;22(10):3292–3300. doi: 10.1128/MCB.22.10.3292-3300.2002

FIG. 1.

FIG. 1.

Association of c-Abl with Rad9. (A) 293T cells were transfected with 3 μg of pSRαMSV/c-Abl and/or pHM6/Rad9. Transfection efficiency as determined with pcDNA3-LacZ and β-galactosidase staining was 81.7% ± 9.2% (mean ± standard deviation of three independent experiments). Cell lysates were immunoprecipitated with anti-HA (left) or anti-c-Abl (right). Immune complexes were subjected to immunoblotting (IB) with anti-c-Abl and anti-HA. (B) Lysates from U-937 cells were immunoprecipitated with preimmune rabbit serum (PIRS), anti-c-Abl, or anti-Rad9. The precipitates were subjected to immunoblotting with anti-c-Abl (top) or anti-Rad9 (bottom). (C) U-937 cells were treated with 10 μM ara-C or 15 Gy of IR and collected at 2 h. Anti-c-Abl immunoprecipitates were analyzed by immunoblotting with anti-Rad9 (top) or anti-c-Abl (bottom). (D) Nuclear extracts from U-937 cells were layered onto a 10 to 35% glycerol gradient. After fractionation, the indicated fractions were subjected to SDS-PAGE and immunoblotting with anti-c-Abl (top) or anti-Rad9 (bottom).