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Inhibition of AMPK kinase activity in RKO cells by treatments that enhance HuR presence in the cytoplasm. Three hours after exposure of RKO cells to either 20-J/m2 UVC (lane UVC) or 1 mM ATP (lane ATP), whole-cell lysates were prepared and AMPK kinase activity was tested after IP with polyclonal antibodies recognizing the AMPK α1 and α2 subunits and with synthetic peptide SAMS as the substrate. −, untreated lysates.
