Figure 11.

Troubleshooting – example of vehicle control impacting cell viability

Pictures demonstrate our vehicle control, DMSO, at two different volumes (that matched the volume present in paired CK-666 experiments), as well as an example of non-treated cells.

Images taken at the lower DMSO concentration match process arborization and cell morphology of non-treated cells.

However, higher concentrations of DMSO result in cells looking more like the drug treated cells already at the beginning of acquisition.

If, for a given mouse, cells in a non-treated tissue slice look normal but then do not in the vehicle control points towards the need to prepare a more concentrated drug to minimize vehicle volume. Scale bars represent 50 μm.