Figure 13.

Troubleshooting – example of too much balance pressure on micropipette preventing cell interaction near micropipette tip

Examples of a chemotaxis assay where ATP was injected alongside the blue Dextran, with cells that were imaged for an hour to watch cell processes extend to interact with the tip of the micropipette.

As can be seen in the top line, too much balance pressure results in a steady deposition of dextran (the blue does not disappear), and processes do not close in on the tip of the micropipette as they do in the bottom row of images (amount of green fluorescence visible within the red circle surrounding the tip of the micropipette).

Scale bars represent 50 μm.