Figure 2.

Workflow for hippocampal slice production

(A) Preparation prior to removing the brain from the mouse. All tools should be laid out (i), cutting solution should be bubbled on ice (ii), the vibratome should be switched on, assembled, and cooling (iii), and the recovery ACSF should be bubbled at 37°C awaiting slices (iv).

(B) The brain is placed in the Petri dish of pre-chilled cutting solution, and a razor blade is used to remove the cerebellum (dotted line).

(C) Using a spatula, the brain is carefully flipped onto a dot of superglue on the vibratome chuck (marked in blue arrowhead).

(D) Chuck is mounted onto a vibratome, and the brain undergoes coronal slicing. 400 μm thick slices are removed via transfer Pasteur pipette (yellow arrowhead) and moved to the container of 37°C ACSF in the water bath.

(E) Slices incubate at 37°C in ACSF for 30 min.