Figure 3.

Preparation of brain slices and micropipette for experiments

(A) Demonstration of CK-666 and DMSO vehicle control-containing ACSF (i), with gas exchange bubbling and slice transfer (ii, iii) (slice indicated with blue arrowhead).

(B) Two-photon microscope set up demonstrating stage (yellow arrowhead), perfusion tubing (pink arrowhead), and micromanipulator for micropipette (green arrowhead).

(C) Using the Pasteur pipet as during vibratome sectioning, adding the slice into the bath on the two-photon stage (red arrowhead).

(D) Creating loading tips (yellow tip heads) and loading micropipettes (white arrowhead) (i), loading the bead mixture into the micropipette (ii), and loading the micropipette into the micromanipulator before positioning it near the tissue slice (iii).