Figure 5.

Example data output

(A) Representative images from the first 30 min of a bead interaction acquisition run demonstrated in Figure 6E.

Baseline can be seen in the first panel (time = 0 min); there are no red beads present. The blue dextran in the pipette is also evident in these maximum intensity projection images. The second panel shows the injection, as there is a blue dextran presence throughout the image.

The following four panels show timepoints at 6-minute intervals after injection, demonstrating the blue dispersing, the beads appearing and settling, and the green microglial processes starting to come in and interact with the beads.

Images were taken every 2 min, but every 6 min are shown here. Scale bars represent 50 μm.

(B) Example image from 2.5X objective size, demonstrating where exactly this specific injection occurred, compared to anatomical landmarks for parts of the hippocampus, such as the CA1 region, CA2, CA3, and dentate gyrus (DG).

Subset (right) shows the end of the micropipette in clearer detail.

Scale bars represent 1 mm (left) and 500 μm (right subset).