Figure 6.

Troubleshooting – examples of reasons the micropipette is not dispensing beads

(i) Representative image of a clump of beads and other debris that has blocked the micropipette further up the length away from the tip.

(ii) Representative image of a clear micropipette with a small opening, resulting in lack of bead dispersion. This size opening would be beneficial for injections that do not require physical particle deposition, such as chemotaxis.

(iii) Representative image of a correctly sized micropipette clear of debris or blockage, ready for injection of beads. Scale bars represent 50 μm.