Figure 9.

Troubleshooting – example time series of cell processes and even cell bodies being pushed away within the imaging area from a very strong injection

High pressure or extended time of injection can result in “blowing away” the cells that are near the micropipette tip.

The red arrowhead in both time series represents where the end of the micropipette tip is located.

In the top series, where there is too much injection pressure, the processes that were near the cells are dispersed and do not recover.

In addition, a couple of cell bodies in the upper half of the field of view vanish after the injection.

In contrast, the bottom series demonstrates depositing beads into the tissue without disrupting or displacing processes or cell bodies of the surrounding tissue. Scale bars represent 50 μm.