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Cutting Solution
| Reagent | Formula Weight | Final concentration (in mM) | Amount (in g/L) |
|---|---|---|---|
| KCl | 74.55 | 2 | 0.15 |
| CaCl2 · 2H2O | 147 | 1 | 0.15 |
| NaH2PO4 | 120 | 1.25 | 0.15 |
| MgSO4 · 7H2O | 246.5 | 2 | 0.49 |
| MgCl · 6H2O | 203.3 | 2 | 0.41 |
| NaHCO3 | 84.01 | 26 | 2.18 |
| D-glucose | 180.2 | 10 | 1.80 |
| Sucrose | 242.3 | 206 | 70.51 |
| ddH2O | N/A | N/A | Up to 1L |
| Total | N/A | N/A | 1L |
Store at 4°C for up to 3 months.
