| Solution Component | Amount (in μL) |
|---|---|
| Cascade Blue labelled 3000MW Dextran (10mg/mL) | 15 |
| iC3b-pHrodo 2 μm beads | 1.5 |
| DMSO or CK-666 200μM (optional) | 0.8 |
| ACSF | 233.5 (or 232.7) |
| Total | 250 μL |
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