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. 2002 Jun;22(11):3577–3589. doi: 10.1128/MCB.22.11.3577-3589.2002

FIG. 8.

FIG. 8.

Expression of BimAD. (A) Immunoprecipitation (I.P.) of lysates from 293 cells after transient transfection of BimEL, BimS, or BimAD or of lysates from untransfected cells. Both polyclonal (poly) and monoclonal (mono) anti-Bim antibodies were employed in the study. Western blot analysis was performed with the polyclonal anti-Bim antibody. Control experiments involved immunoprecipitation with preimmune serum (pre-imm.), blocking of the anti-Bim polyclonal antibody with a specific peptide (+pept.), or immunodepletion of 293 cell lysates with the polyclonal antibody (imm.depl.). (B) Western blot of whole-cell lysate from 293 cells or 293 cells transiently transfected with BimEL or BimAD as controls. The expression of Bim was detected by immunoblotting with an anti-Bim polyclonal antibody. (C) Expression of Bim isoforms in Jurkat, HelaS3, HL60, and Daudi cells. (D) Immunoprecipitation of endogenous Bim from Daudi and HL60 cells and of exogenous Bim in transfected 293 cells with either a polyclonal antiserum (PoAb) or a monoclonal antibody (MoAb). The presence of Bax in the immunoprecipitated complexes was detected with the polyclonal Bax N20 antiserum. IgG, immunoglobulin G.