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. 2002 Jun;22(11):3744–3756. doi: 10.1128/MCB.22.11.3744-3756.2002

FIG. 2.

FIG. 2.

Gab3, but not Gab2, interacts with Mona in vivo. (A) Cell lysates from FD/Fms/Mona cells were immunoprecipitated (IP) using anti-Mona or anti-Gab2 antibody. Whole cell lysates (WCL; 50 μg) and immunoprecipitates were separated by SDS-12% PAGE and immunoblotted (IB) with anti-Gab2 (1:2,000), anti-Mona (1:1,000), or anti-Grb2 (1:5,000) antibody. (B) Cell lysates from FD/Fms/Gab3V5/Mona and FD/Fms/Gab3V5 cells were immunoprecipitated using antibodies against Mona or the V5 epitope detecting Gab3. WCL (50 μg) and immunoprecipitates were separated by SDS-12% PAGE and then immunoblotted with anti-V5 (1:5,000), anti-Mona, or anti-Grb2 antibody.