FIG. 5.
M-CSF regulates Gab3 phosphorylation and association with Mona. (A) FD/Fms/Gab3V5/Mona cells were washed in IMDM, starved, and then stimulated (except the zero time point) with 2,500 U of M-CSF per ml for the indicated times at 37°C. Cell lysates from each time were subjected to immunoprecipitation (IP) using anti-V5 antibody. Immunoprecipitates were separated by SDS-12% PAGE and proteins were visualized by immunoblotting (IB) using antiphosphotyrosine (αpY; 1:10,000) or anti-V5 (1:5,000) antibody. (B) As for panel A, but proteins in cell lysates were immunoprecipitated with anti-Mona antibody. Immunoprecipitates were separated by SDS-12% PAGE and visualized by immunoblotting using anti-V5, antiphosphotyrosine, or anti-Mona (1:1,000) antibody.